Sentence examples for buffer were shaken for from inspiring English sources

The samples with 10 mL reaction buffer were shaken for 48 h at 50°C as the control.

The samples with 6 mL of reaction buffer were shaken for 48 h at 50°C as the control.

The membranes were immersed in blocking buffer and a corresponding IgG-conjugated secondary antibody and were shaken for 1 hr.

The samples were shaken for 5 min, and 1.25 ml of Tris HCl buffer mixture (pH 7.5, 50 mM) was then added (containing 1 M NaCl).

Fresh apo wtAidB (200 nM to 5 μM) and 1 μM FAD, each in AidB buffer, were mixed by being shaken for 2 min. Fluorescence was recorded over time until equilibrium was reached.

35 μl benzonase buffer containing 0.1 Unit/μl benzonase gradeII (Merck, Darmstadt, Germany) is added and plates are shaken for 30 sec followed by a 30 min incubation on the cooling plate and another 30 sec shaking step.

For chemotaxis, 5×10 cells/ml were shaken in KK2 buffer for 5 hours while being pulsed every 6 minutes with cAMP to a final concentration of 10-7 M. The cells were then placed in the Zigmond chamber (Z02; Neuro Probe, Gaithersburg, MD) in a cAMP gradient (source at 10-6 M cAMP, sink with no cAMP).

The process was performed in the presence of 50 mM citrate buffer (pH 5) at 50°C and samples were shaken at 150 rpm for 72 h.

Cultures were shaken (600 rpm for 5 minutes) and non-adherent cells removed by two washes in phosphate buffered saline (PBS).

FastPrep tubes containing the frozen tissue plus Lysing Matrix beads were shaken in the homogenizer (FastPrep) for 40 s at speed 6 (6 m/s) without buffer at room temperature.

After addition of magnesium sulphate, sodium chloride and citrate buffer, the mixture is shaken intensively and centrifuged for phase separation.