Sentence examples for buffer were separated by from inspiring English sources

The proteins eluted with boiled SDS-PAGE sample buffer were separated by SDS-PAGE.

Briefly, unheated samples in Laemilli buffer were separated by SDS/PAGE, blotted onto PVDF membranes and then blocked in 5% non-fat milk.

Proteins suspended in Laemmli buffer were separated by SDS-PAGE (10% gels), transferred onto PVDF membranes (Millipore, US) and revealed with specific antibodies.

For immunoblot experiments, 20 to 50 μg of lysates in SDS loading buffer were separated by SDS-PAGE.

Denatured protein samples with 1 × loading buffer were separated by 12%% polyacrylamide gel electrophoresis followed by transfer to a pure nitrocellulose blotting membrane (Pall Corporation).

Proteins extracted using RIPA buffer were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), electrotransfered and immobilized on a nitrocellulose membrane.

0.4 uL of each individual serum sample (non-diabetic and diabetic subjects, n = 24, respectively) diluted to 1/10 with 1.0 M Tris (pH 6.8) buffer was separated by SDS-PAGE, and electro-transferred to a nitrocellulose membrane (Whatman International Ltd., England).

Cell lysates (45 μg protein) in SDS sample buffer were separated on 7% Tris-acetate gel by SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes.

The bound proteins were eluted with SDS loading buffer and were separated by SDS/PAGE followed by immunoblotting with antibody against STAT3 (Santa Cruz Biotechnology, Dallas, TX, USA).

Cell lysates were boiled in Laemmli sample buffer and were separated by SDS-PAGE, transferred to nitrocellulose filters, probed with the antibody of interest, and developed using ECL (Amersham Biosciences, Pittsburg, PA, USA).

After washing and elution in SDS-containing loading buffer, proteins were separated by SDS-PAGE.