Sentence examples for buffer were run at from inspiring English sources

NuPAGE Bis-Tris Gels (4% to 12%; Invitrogen) in 3- N-morpholino propanesulfonic acid buffer were run at 175 V for 1.5 hours, and proteins were transferred at 3- N-morpholino propanesulfoniclidene fluoride membranes.

Agarose gels (0.8%, 12 cm) cast in 10 mM sodium phosphate buffer were run for 2 hours at 100 volts with the buffer recirculated by a peristaltic pump.

Samples were analyzed by FPLC using an AKTA Explorer Superose™ 6 size-exclusion column (Amersham Pharmacia) in which particles suspended in 0.1 M phosphate buffer (pH 7.0) were run at a rate of 0.4 ml/min and absorbance was measured at 260 and 280 nm.

Then, the cathode buffer was exchanged for cathode buffer B/10 (buffer B but containing only 0.002% Coomassie Blue G250) and gels were run at 100 V [anode buffer: 50 mM Bis-Tris (pH 7)].

The reaction products were separated on a 1% agarose gel in Tris-borate-EDTA buffer, containing SYBR safe (Invitrogen), and were run at 100 volts.

Gels were run at 100V for 3.5 to 4 hours with 1× native buffer supplemented with 1 mM ATP.

All gels were run at 40 mA using a mini Protean apparatus (BioRad) and Laemmli buffer.

Gels were run at a constant 175 V for 90 min at 4°C in a buffer containing 25 mM Tris and 192 mM glycine buffer.

Formaldehyde-MOPS gels were run at 100 volts for loading and 50 volts overnight in 1 × MOPS buffer.

All gels were run at 200 V constant for 50 min in 3- N-morpholino propanesulfonic acid (MOPS)-SDS buffer.

The gels were run at 200 V, 60 W per gel, 45 min running time, using 1 × MES SDS running buffer.