Sentence examples for buffer were resuspended in from inspiring English sources

The pellets resulting from centrifugation after homogenization in RIPA buffer were resuspended in urea buffer (7 mol/L urea, 2 mol/L thiourea, 4% CHAPS), sonicated, and centrifuged.

After extensive washing in lysis buffer, precipitates were resuspended in SDS sample buffer, resolved by SDS-PAGE, and analyzed by Western blotting employing standard methods.

The cells were washed with modified Tanaka buffer and were resuspended in the same buffer containing 5 μM of DAPI and 1 mM 2,4-dinitrophenol (DNP), and incubated at 37°C for 10 h [ 27, 28].

After washing twice with the wash buffer, cells were resuspended in 100 µl wash buffer, transferred in a microtiter plate and fluorescence intensity was measured at excitation 485 nm and emission 530 nm.

After four washes for 5 min each with 1 mL IP buffer, beads were resuspended in 2X-SDS Laemmli buffer, followed by SDS-PAGE and immunoblotting.

The enriched IE were washed twice with panning buffer and were resuspended in 10 ml of panning buffer at a concentration of approximately 5×107 IE/ml.

The pellets were then washed twice with HEPES buffer and were resuspended in 100 µl of the same buffer.

Then cells were washed with the FACS buffer and were resuspended in each tube with 500 μl of FACS buffer for FACS analysis.

After washing with cold buffer, cells were resuspended in 300 μl of PBS for FACS analysis by FACScan (Becton Dickinson).

After washing two times with protein lysis buffer and once with PTP reaction buffer the precipitates were resuspended in 40 μl PTP reaction buffer.

Cells were lysed in NP-40 lysis buffer, and nuclei were resuspended in MNase digestion buffer.