Sentence examples for buffer were resolved by from inspiring English sources
Brain, liver, and spleen homogenates (10%) prepared in lysis buffer were resolved by SDS-PGE and subjected to western blotting using specific antibodies as described in previous reports [30].
Cell lysates extracted in RIPA buffer were resolved by 3 8% SDS-PAGE.
Proteins in Laemmli sample buffer were resolved by SDS-PAGE (10%) and transferred to PVDF Immobilon-P membrane.
Cells lysates, prepared by dissolving washed cells directly in SDS sample buffer, were resolved by SDS-PAGE with indicated percentage of acrylamide.
Whole-cell protein extracts prepared in Laemmli sample buffer were resolved by SDS-PAGE using 4% to 12% polyacrylamide gels (NuPage; Invitrogen Corp ., transferred to PVDF membranes (Invitrolon; Invitrogen Corp ., and probed with primary and horseradish peroxidase-conjugated secondary antibodies.
Whole-cell protein extracts prepared in Laemmli sample buffer were resolved by SDS-PAGE using NuPage 4 12 % gels (Invitrogen), transferred to Invitrolon polyvinylidene difluoride membranes (Invitrogen), and probed with primary and horseradish peroxidase-conjugated secondary antibodies.
For WB of IGF-I (Fig. 5), 1.0 μL plasma samples diluted in reducing Laemmli buffer was resolved by PAGE (4 20% Criterion TGX Bio-Radd) and transferred to PVDF membranes.
The beads were washed with Co-IP buffer, and captured proteins eluted from the beads using loading buffer was resolved by SDS PAGE for western blot analysis using a monoclonal TDP-43 antibody (Proteintech, 60019-2-Ig).
20 μg to 30 μg of total protein in SDS sample buffer was resolved by SDS-PAGE (10% or 12.5%) and transferred to PVDF membrane using a semi-dry transfer system (Hoepher SemiPhor).
Equal amounts of protein in Laemmli sample buffer [44] were resolved by SDS-PAGE and electroblotted onto nitrocellulose membranes.
Tumor lysates were prepared by homogenization of tumor tissues in RIPA lysis buffer and were resolved by SDS-PAGE and transferred onto PDVF membranes and immunoblotted with anti-ME2 antibody and normalized by β-tubulin as a loading control.
