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Spike-in controls oligonucleotides, negative control and buffer were printed in quadruplicates onto each subarray.
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Elements were printed in a 150 mM sodium phosphate printing buffer (pH 8.5).
PNGase F was printed over the right half of a section, while buffer was printed over the other half at spacing of 100 μm as a negative control.
Significant data were printed in bold.
The probes were printed in duplicate.
All questionnaires were printed in Chinese.
All oligonucleotides were printed in quadruplicates over 4 subarrays with a 2 × 2 print head.
The original extracts, plus two dilutions (1 2 and 1 5) were printed in triplicate (printing replicates).
Consent forms were printed in the three official languages.
All brochures were printed in color on A4-size paper.
The booklets were printed in B5 size (176×250 mm).
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