Sentence examples for buffer were prepared with from inspiring English sources

For the experiment shown in Fig. 7A, two identical CaM-488/CKII peptide samples in buffer were prepared, with one containing an addition of ascorbic acid during the photo-unbinding step at a concentration of 8 mM (pH adjusted to 7.2 by titration with HCl).

Blank solutions consisting of CD buffer were prepared with and without 100 mM glucose.

Borate buffer was prepared with a required ratio of 50.0 mM sodium borate (Na2B4O7·10H2O) to 0.20 M boric acid [35].

It is recommended that the 15% denaturing PAGE gel containing 7 M urea and TBE running buffer be prepared with DEPC treated water.

Isoelectric focusing (IEF) buffer was prepared with 2% (volume/volume) ampholyte (pH 3.7 9.2).

Cell staining and flow cytometry analysis staining buffer was prepared with PBS (Phosphate buffered saline, without Ca2+ and Mg2+, EuroClone Ltd., Torquay, UK) supplemented with 2,5% (vol/vol) FCS and 0,01% (vol/vol) sodium azide.

POP stock solutions were prepared at a concentration of 200 ngr/µL in Tris HCl 50 mM pH 8. Stock solutions for the substrate were prepared in H2O : MeCN (1∶1, v/v) for each peptide, and the activity buffer was prepared with Na2HPO4 and KH2PO4 until pH 8 was reached.

Assay buffer was prepared with PBS, 0.05% (v/v) Tween20, and 1% BSA.

All assays were conducted at 37°C in TNC buffer [50 mM Tris/HCl, 150 mM NaCl, 10 mM CaCl2, 0.05% Brij-35 and 0.02% sodium azide). This buffer was prepared with a pH of 7.70 at 20°C, giving a pH of 7.30 at 37°C. Assays were conducted in sealed 0.5 ml microfuge tubes with endpoint fluorescence measured after 18 h using a Gemini microplate spectrofluorometer (Molecular Devices).

Throughout this study, buffers were prepared with apyrogenic water obtained from Braun Medical (Boulogne, France).

CD98 was purified from the urea soluble fraction by elution off a HisBind Resin column following the protocol of the HisBind Purification kit (Novagen) with the following modifications: buffers were prepared with the addition of 6 M urea.