Sentence examples for buffer were precipitated with from inspiring English sources
The proteins present in each extraction buffer were precipitated with trichloroacetic acid (TCA) (Fisher Scientific, Fair Lawn, NJ), washed twice in cold acetone, and resuspended in an equal amount of Lammli buffer [30].
Then the cells were lysed in RIPA buffer and medium proteins were precipitated with 70% acetone as indicated above.
The proteins in the filtered-culture supernatants were mixed with 1/5th of the volume of SDS-PAGE sample buffer (5X) for WB or were precipitated with trichloroacetic acid (TCA 20% w/v; Merck) for Coomassie staining.
Total protein was extracted with SDS-lysis buffer, and culture supernatant (CS) proteins were precipitated with 10% TCA.
Samples were washed and homogenized in 1 × passive lysis buffer (Promega, Fitchburg, WI, USA), and proteins were precipitated with 25% trichloroacetic acid (TCA) (Sigma, Gillingham, UK).
Proteins were precipitated with protein A-sepharose beads, washed three times in lysis buffer, and eluted with sample buffer.
Potential MBP-TRIAD1/CUL5 RBX2 MBP-TRIAD1/CUL5 RBX2pitated with amylose resin and washed with reacomplexesfer.
Proteins from each aliquot were precipitated with trichloroacetic acid and resuspended in SDS-PAGE sample buffer.
Supernatants were precipitated with trichloro acetic acid (TCA) and resuspended in SDS sample buffer (Invitrogen, Life Technologies).
All fractions were precipitated with trichloroacetic acid, washed with acetone, dried, resuspended in loading buffer, and analyzed by western blot.
