Sentence examples for buffer were precipitated by from inspiring English sources
Briefly, for the 1D PAGE, samples boiled in 2X electrophoresis sample buffer were precipitated by 5-fold volume of pre-chilled methanol at −20 °C for 2 h, followed by centrifugation at 14,000 rpm for 20 min at 4°C.
After pooling and diluting purified VP1-containing fractions in DB150 buffer, VP1-derived VLPs were precipitated by ultracentrifugation for 4 h at 100,000 × g.
The LATEX beads were precipitated by centrifugation and washed twice with a high pH buffer and once with a high salt buffer to remove the contaminant proteins.
The beads were precipitated by centrifugation steps and washed five times in NP-40 buffer before sodium dodecyl sulfate loading buffer was added.
Immune complexes were precipitated by centrifugation, followed by extensive washing in lysis buffer.
The input bacterial extract sample and 1M NaCl eluates were precipitated by methanol-chloroform and re-solubilized in SDS-sample buffer prior to separation by SDS-PAGE.
The proteins in the supernatant were precipitated by dissolving ammonium sulfate and the precipitate was dissolved in 50 mM acetate buffer (pH 6.0).
After the proteins were precipitated by adding of Na2SO4 (1.27 M) to the serum, the precipitate was resuspended in phosphate buffer (0.02 M NaH2PO4, 0.02 M Na2HPO4, pH 7.0) and dialyzed thoroughly against the same buffer.
For Western analyses, 500 μl fractions were precipitated by TCA (trichloroacetic acid), washed in acetone, resuspended in 100 μl of onefold SDS sample buffer and separated by SDS-PAGE.
