Sentence examples for buffer were preadsorbed with from inspiring English sources

In parallel, 20 µl of a 50% slurry of protein G Sepharose in RIPA buffer were preadsorbed with 5 µl polyclonal rabbit anti-GFP antiserum (Invitrogen) and incubated for 2 h at 4°C.

A well-organized focal adhesion complex and actin stress fibers were observed only for COOH- and NH2-SAMs when SAMs were preadsorbed with Vn and BSA.

Sera from two randomly selected patients with CD and anti-neural antibodies, were preadsorbed with recombinant h-TG2.

This signal was absent when preimmune serum was used or when antibodies were preadsorbed with 20-fold molar excess of the antigen by western-blot or immunofluorescence (Fig. S1B-C).

For this purpose, selected seropositive samples were preadsorbed with clinical isolates (LAM10406 and H12425) or mock preadsorbed (HSA-coated Latex Beads) before ELISA assay.

Serum samples used for sensitivity and specificity determination (section 2.8) were preadsorbed with pellet, heat denatured and non-heat-denatured cell lysates of only E. coli XL1-Blue MRF'.

To avoid cross-reactions in double and triple labelings with primary goat antibodies, sections were blocked with chicken serum and the other secondary antibodies were preadsorbed with chicken serum as previously described (Schmidt et al., 2004).

Plasma samples were preadsorbed with equivalent (6 ×10 PFU/mL) concentrations of H2O2-inactived monkeypox or vaccinia whole-cell lysate at a 1 30 dilution (5 μL plasma in 145 μL viral lysate) for 30 min at 37°C.

Furthermore, sections were incubated with antibody that had been preadsorbed with KLH conjugated to AmOA1 peptide that was used to make antibodies in goat (Fig. 1G).

Furthermore, sections were incubated with antibody that had been preadsorbed with glutaraldehyde treated KLH (KLH-G) alone (Fig. 1C) or KLH-G conjugated to the AmOA1 peptide used to make antibodies in rabbit (Fig. 1D).

Similarly, in our preliminary ICWs, the signal from resting and stimulated cells was reduced to background levels when the antibody toward PMLC20 was preadsorbed with the same blocking peptide.