Sentence examples for buffer were performed for from inspiring English sources

Control experiments of titrant into buffer were performed for all titrants.

Reverse transcription reactions using SuperScript II (Invitrogen) in the manufacturer's recommended buffer were performed for 50 minutes at 42°C using twice the recommended concentration of enzyme, 1 μl of Protector RNAse inhibitor (Roche) to avoid RNA degradation, 2 μl 5' primer (12 μM), 2 μl 10 mM DTT, and 1 μl 10 mM dNTPs.

The incubation with 50 mM TRIS-HCl, pH 7.8, 10 mM CaCl2 buffer was performed for 19 h at 37°C.

Secondary antibody incubation (streptavidin-allophycocyanin) in flow buffer was performed for 30 minutes on ice.

Immediately after exsanguination, a bronchoalveolar lavage (using 40 mL sterile saline buffer) was performed for further biochemical investigations.

Incubation with primary antibodies (supplementary material Table S4) in dilution buffer was performed for 1 hour at room temperature.

Subsequent to the incubation stage, a buffer exchange with α-Syn-free buffer was performed for 6 s which removed unbound α-Syn.

Incubations with primary and secondary antibodies, diluted in PBS buffer, were performed at 4°C overnight and at RT for 60 min, respectively.

No template controls (buffer blanks) were performed for each amplification method.

For C. albipunctata embryos, the following modifications to the staining protocol apply: proteinase K treatment was carried out for 7 min at room temperature; post-hybridization washes: an additional wash of 10 min 2xSSC/hybridization buffer was performed before washing for 15 min with PBT/hybridization buffer; antibody incubation: embryos were incubated with anti-DIG for 2 hrs.

To assess cell viability, 4 ′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes-Invitrogen, 1 : 1000 in phosphate buffered saline (PBS)) stainings were performed for 1 min at room temperature.