Sentence examples for buffer were performed at from inspiring English sources

Incubations in deuterated buffer were performed at intervals from 10 s to 1 hr.

Careful rinses with several changes of TBS-0.3% tween buffer were performed at each step.

Incubations with primary and secondary antibodies, diluted in PBS buffer, were performed at 4°C overnight and at RT for 60 min, respectively.

Migration in Tris glycine buffer was performed at 10 mA for 120 minutes and then at 20 mA for 60 minutes, using the Mini-Protean II® cell system from BioRad (CA, USA).

Electrophoresis in Tris borate ethylenediaminetetraacetic acid buffer was performed at 100 V for 1.5 h.

Electrophoresis in Tris-borate-EDTA buffer was performed at 100 V for 1.5 h.

Electrophoresis in Tris-borate-EDTA buffer was performed at 120 V for 35 min.

Hybridization with the αP-dCTP-labeled probe at a final concentration of 10cpm/mL of hybridization buffer was performed at 42°C overnight.

Reverse transcription reactions using SuperScript II (Invitrogen) in the manufacturer's recommended buffer were performed for 50 minutes at 42°C using twice the recommended concentration of enzyme, 1 μl of Protector RNAse inhibitor (Roche) to avoid RNA degradation, 2 μl 5' primer (12 μM), 2 μl 10 mM DTT, and 1 μl 10 mM dNTPs.

The cellular Tyrosinase and L-DOPA solution (0.1 M in sodium phosphate buffer) reaction were performed at 37 °C for 1 h.

Incubation with primary antibodies diluted in blocking buffer was performed overnight at room temperature (RT).