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Concentration and composition of the starting buffer were optimized as well.
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Branch lengths and rate parameters were optimized as well.
During purification, process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification.
These aspects were optimized as separate parameters.
RRM4 and poly(A) relational geometries were optimized as above.
Finally, suitable extraction conditions were optimized as follows.
Therefore, the mass of methanol is optimized as 60 g.
Our NGAL ELISA was optimized as follows.
The washing procedure and the buffer composition were optimized to achieve maximal sperm viability for the duration of the experiment (see Material and Methods).
The ionic strength of buffer solutions was optimized and kept as low as possible to support protein complex formation as well as protein solubility (see Figure 7 of ref (62)).
Several parameters that influenced CE separation quality were optimized, such as buffer type, pH value, concentration and applied voltage.
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