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Extracts of BicD::GFP embryos in RIPApi buffer were obtained as described above, and incubated with the anti-GFP beads over night at 4°C with constant mixing.
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Only random and sparse CNTs in the samples without and with a 20-nm Au buffer layer were obtained, as shown in Figure 2a, b.
In the optimization studies of the biosensor, glycine buffer (pH 10.5; 50 mM) and 30 °C were obtained as the optimum working conditions.
Tissues were homogenized in ice-cold lysis buffer, and cytoplasmic and nuclear proteins were obtained as described [ 8].
Briefly, rat brains were obtained as above and homogenized in Buffer A (0.32 M sucrose, 1 mM NaHCO3, 1 mM MgCl2, 0.5 mM CaCl2).
RIPA buffer was obtained from Santa Cruz (Heidelberg, Germany).
Sterile phosphate buffered saline (PBS) buffer was obtained from Life Technologies.
RIPA buffer was obtained from Millipore.
RIPA buffer was obtained from Biosesang Inc. (Seongnam, Korea).
High-resolution (49 μm) maps of fluid density and spin lattice relaxation rate were obtained as a function of time for CPP immersed in phosphate buffered saline.
Spectra were obtained as above.
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