Sentence examples for buffer were not used from inspiring English sources

Water and buffer were not used for dilution since either of these diluents would have altered the lysate matrix concentration, to falsely influence the PLS regression model.

Due to a number of factors, a buffer was not used for the reactions described above.

For part of the samples, the red blood cell lysis buffer was not used.

Mitochondria were purified using sucrose gradients as previously described [32], except that DTT-containing wash buffer was not used.

Hybridization and washing were performed following the "Two-colour microarray based gene expression analysis" protocol from Agilent, with the following exceptions: fragmentation buffer was not used, 1 µl of a mixture of labeled oligonucleotides was added, and the mixture of cDNA was incubated at 98°C for 3 minutes for denaturing.

Sodium acetate buffer was not used for the serum samples.

We found that the photo-switching of Alexa 750 in buffer containing MEA was not robust, with a large fraction of fluorophores photo-bleaching after a small number of switching cycles, and hence this imaging buffer was not used for Alexa 750 STORM imaging.

Use of lower pH buffers were not used in order to avoid loss in biologic activity of the HSP72 ligand.

Low-spin fractions could only be eluted with 200 mM phosphate buffer, pH 8.0, and were not used for further work.

Caspase-3 was not used in assay buffer to blanks.

If citrate accumulates, iCa decreases and metabolic acidosis ensues, since bicarbonate continues to be removed by filtration or dialysis, while citrate is not used as a buffer.