Sentence examples for buffer were mixed with from inspiring English sources

Equal amounts of proteins (∼3 mg) in 600 µl of lysis buffer were mixed with an equal volume of 80% sucrose and laid at the bottom of ultracentrifuge tubes.

Extracts (500 µg protein/ml buffer) were mixed with 60% Optiprep™ (Nycomed, Denmark) to reach a final concentration of 40% and overlaid in a SW40 centrifugation tube (Beckman, CA, USA) with a step gradient of 30 and 5% Optiprep™ in MES-buffer.

Two microliters of the sample buffer were mixed with 4 μL of PCR product.

A total of 200 μL of lysis buffer were mixed with the pellets and incubated on ice for 30 minutes.

10 μl of DNA samples diluted in 90 μl of 1x TE buffer were mixed with 100 μl PG working solution to reach a final volume of 200 μl.

Aliquots of 10 μM to 900 mM MgCl2 in the annealing buffer were mixed with the sample by manual pipetting; the sample was equilibrated at 25 °C in a recirculating water bath for 5 min before each measurement.

Briefly, 50 μl cell lysis buffer was mixed with 10 μl Ac-DEVD-pNA (2 mM) and 40 μl buffer and loaded into a 96-well plate.

In each run 0.1 mL of cell suspension (1 10 dilution in the resuspension buffer) was mixed with an equal amount of iso (baseline) or hyperosmotic solution (sorbitol or glycerol 2.1 M, 50 mM K-citrate buffer pH 5.1 or pH 7.4) of 1.25 tonicity ((Λ = (osmout)∞/(osmout)o)).

For enzyme extraction, 20 mL of the sodium acetate buffer was mixed with the solid medium (200 mM, pH 6.5).

For enzyme immobilization, 1 ml of lipase solution (1.0 mg ml−1 of lipase in 50 mM, pH 8.0 Tris HCl buffer) was mixed with 18 mg of NPG.

Blocking buffer (2.5% SDS, 20 mM MMTS in HEN buffer) was mixed with the samples and incubated for 30 min at 50°C to block free thiol groups.