Sentence examples for buffer were mixed in from inspiring English sources

Tissue and buffer were mixed in a 1 20 relation.

Tissue and buffer were mixed in a 1 20 ratio.

Substrate and buffer were mixed in a 96-well plate prior to addition of protein by the plate reader.

First, 20 μL of 15 mM Na2EDTA in buffer (50 mM KH2PO4/KOH, pH 7.4), 50 μL of 0.6 mM NBT in buffer, 30 μL of 3 mM hypoxanthine in 50 mM KOH, 5 μL of the test samples in methanol, and 145 μL of buffer were mixed in 96-well microplates (Falcon, USA).

500 ul type I collagen gel solution (0.3%) containing 5 mg/ml of human fibronectin (Collaborative Research Inc. Lexingtonf, USA), 100 ul 10 × DMEM and 400 ul NaOH-Heppers buffer was mixed in an ice cold condition and pipetted into a 6-well plate and kept for 30 min at 37°C or gelatinization.

Type I collagen, 10× concentrated F-12 medium, and reconstitution buffer were mixed together in ice water with a ratio of 8 1 1 (Primaster® content).

Aliquots of 10 μM to 900 mM MgCl2 in the annealing buffer were mixed with the sample by manual pipetting; the sample was equilibrated at 25 °C in a recirculating water bath for 5 min before each measurement.

Preassembled triangles (5 μM each in TMS buffer) were mixed with urea (8 M final concentration) and either incubated at room temperature (RT) for 30 min or heated to 100 °C followed by cooling on ice.

Briefly, ~2.0 μg PCR products in QIAGEN EB buffer were mixed with an equal volume of 2x Binding and Wash buffer (1x buffer: 5 mM Tris, pH7.5, 0.5 mM EDTA, 1.0 M NaCl).

Briefly, a 0.5 mL solution of 0.1 mg/mL phenol-red in phosphate buffer (pH 7.4; 0.1 M) and 50  μg/mL horseradish peroxidase in the same buffer were mixed with 0.5 mL of different skin supernatant fractions and incubated at 37°C for 10 min.

The two buffer stocks were mixed in different ratio providing nearly linear dependence of the pH on the mixture composition (see Fig. 9).