Sentence examples for buffer were mixed at from inspiring English sources

CSF, Cibacron Blue/Protein A gel slurry, and binding/wash buffer were mixed at 2 1 2 (v/v/v) ratio.

300 ng of Cy5 and Cy3 labeled aRNA in 19 μl volume, 5 μl 10 × blocking agent and 1 μl 25 × fragmentation buffer were mixed together and incubated at 60°C for 30 min. Twenty-five microliters 2× GEx hybridization fuffer was added to each sample to stop the fragmentation reaction.

Tissue and buffer were mixed in a 1 20 relation.

Tissue and buffer were mixed in a 1 20 ratio.

Excess AlkA and 100 nM (final concentration) 19AεA or 19TεC DNA (in glycosylase assay buffer with no BSA) were mixed at 25 °C.

Blocking buffer (2.5% SDS, 20 mM MMTS in HEN buffer) was mixed with the samples and incubated for 30 min at 50°C to block free thiol groups.

Purified H2A and H2B were mixed at a 1 1 molar ratio in unfolding buffer, and the mixture was dialyzed against refolding buffer.

Briefly, 1 µl of 10× Fragmentation Buffer was mixed with 10 µl of DNA solution and incubated for 4 min at 95°C.

SDS-PAGE Sample Loading Buffer was mixed into the supernatant by proportion and the mixture was bathed for 5 min at boiling water.

The protein solutions were mixed at a 1 1 (v/v) ratio with the sample buffer (0.125 M Tris HCl, pH 6.8, 4% SDS, 20% glycerol).

DNA complexes were formed via a thermal annealing procedure: strands were mixed together at a 1 1 stoichiometry in TAE/Mg2+ buffer at a final concentration of 3 μM.