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Fluorescently labelled, purified recombinant proteins in microinjection buffer were microinjected into adherent cells with Femtotips II microcapilliaries using a Femtojet microinjector coupled to an Injectman micromanipulator (Eppendorf) essentially as described [ 34] and according to the manufacturer's instructions.
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The integration into microinjected BPES embryonic stem cells was verified by PCR, and the cells were microinjected into C57BL6/J blastocysts.
To prove our concept, the PPC/DNA complexes were microinjected into HepG2 cells.
OX1R agonist (orexin-A) and antagonist (SB-334867-A) were microinjected into Vc prior capsaicin administration.
TALEN mRNAs were microinjected into the cytoplasm of one-cell Wistar rat embryos.
Naked DNA, polymer only, and polymer/DNA complexes (C/A = 0.5, 1.0, and 2.0) were microinjected into HepG2 cells and GFP expression was detected 24 h after injection.
Positively identified clones were microinjected into C57BL/6 blastocysts that were then implanted into pseudopregnant females.
Positive clones were microinjected into C57Bl/6 blastocysts and transferred into CD-1 foster mothers.
To test these phosphorylation-resistant Mad constructs, mRNAs were microinjected into Xenopus embryos.
RNA and morpholino antisense oligonucleotides were microinjected into the yolk of one-cell stage embryos.
Two targeted ES cell clones were microinjected into C57BL/6 (albino) blastocysts.
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