Sentence examples for buffer were lysed in from inspiring English sources

Cells (1 × 10 cells/50  μL lysis buffer) were lysed in buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 5 mM EDTA, Protein Inhibitor Cocktail (ROCHE) and 1% Triton X-100 for 60 min at 4°C.

After culturing in culture medium or metabolic inhibition buffer, HUVECs were lysed in buffer containing 0.01%NP-40NP-405 mM Tris HCl, (pH 8.0), 150 μM NaCl, and 5 mM EDTA in H2O.

Cells were lysed in buffer containing PBS, 1% Triton and protease cocktail (Roche, Germany).

Cells were lysed in Buffer RLT (Qiagen).

Macrophages were lysed in buffer containing 1%Nonidet-P40Nonidet-P40ted with complete protease inhibitor 'cocktail' (Roche).

Cells were lysed in buffer (9803, Cell Signaling) containing complete protease/phosphatase inhibitor cocktail (78410, Life Technologies).

Cells were lysed in buffer A containing 1 U of RNaseOUT (Invitrogen, Grand Island, NY, USA).

For Western blot analysis, the liver tissues were lysed in buffer containing complete protease inhibitor cocktail.

The cells were lysed in a buffer containing guanidine isothiocyanate (RLT buffer, QIAGEN, CA, USA).

For total cell extracts, cell were lysed in buffer-A supplemented with 3% SDS.

Cells were lysed in RIPA buffer (strong) (biyuntian, China).