Sentence examples for buffer were loaded into from inspiring English sources

About 100 µg protein, extract of 1×106 cells in 350 µl solubilization buffer were loaded into an IPG strip by in-gel re-swelling overnight at RT under silicon oil in a nitrogen- and water-saturated atmosphere to prevent oxidation of the protein and drying of the gel strips.

Human IgG standard (Sigma) and culture supernatants diluted with a blocking buffer were loaded into each well and treated with peroxidase conjugated goat anti-human IgG (Sigma) in the blocking buffer.

110 µL of sample was loaded into the sample sector and 120 µL of buffer was loaded into the reference sector.

In short, equal volumes (15 μL) of the sample in Laemmeli buffer was loaded into each well and resolved.

150 μl EP buffer was loaded into the sample loading port using a P1000 pipette and left for 10 minutes to prime the flowcell.

An extracellular buffer was loaded into the PPC plate wells (11 μL/well) and a cell suspension was added into the wells (9 μL/well).

The CE run buffer was loaded into a sample vial that was then sealed to allow a N2 back pressure to be applied to the buffer liquid, as shown in Figure 1A.

For isoelectrofocusing (IEF), 140 μL of sample solution containing around 30 μg total protein, 18.2 mM dithiotreitol (DTT), and 0.5% immobilized pH gradient buffer solution were loaded into 7 cm non linear pH 3 10 strips.

After cross-validation of the biosensing performance of the LSPR platform with the ELISA technique, THP-1 cells suspended in buffer solution were loaded into the LSPR device for on-chip cell separation, incubation, stimulation, and detection of cell-secreted TNF-α.

The CFD was removed, a cap was placed over the DNA, and reagents (antifade, buffer, enzyme, stain) were loaded into the MapCard reservoirs.

Equal amounts of protein and Laemmli buffer (Bio-Rad) were loaded into an 18-well, pre-cast polyacrylamide 4 15% gradient gel (Bio-Rad).