Sentence examples for buffer were isolated as from inspiring English sources

ER microsome-targeted RNCs in membrane buffer were isolated as described above and treated with 50 μM bis-maleimidohexane (BMH, Pierce, Rockford, IL) or bis-maleimidoethane (BMOE, Pierce) for 30 min at 0°C.

Mice were anesthetized, transcardially perfused with phosphate buffered saline (PBS), and CNS cells were isolated as described previously [20], with the following adjustments.

Adipose tissue was washed in PBS (phosphate buffered saline, PAA Laboratories GmbH, Pasching, Austria and ASCss and mature adipocytes were isolated as described before [ 19].

Cells were lysed with M-PER buffer (Thermo) containing protease and phosphatase inhibitors (Sigma); nuclear/cytosolic fractions were isolated as previously described (Settembre et al, 2011).

Nuclei were isolated as described above and resuspended in HERR buffer (20 mM HEPES pH 7.9, 150 mM KCl, 0.1% NP40, 10% glycerol, 2 mM EDTA).

Nuclei were isolated as described above, excluding formaldehyde crosslinking and the addition of sonic buffer.

Then the lysates were isolated as the Triton-soluble fraction, and the pellets were resuspended in 2× SDS loading buffer as the Triton-insoluble fraction or in buffer appropriate for other assays.

LPMC were isolated as previously described [42].

Murine cardiomyocytes were isolated as described [20].

Cell wall lipids were isolated as above.

were isolated as previously described [17].