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The 10 μM kinase inhibitor standards, dissolved in 25% ACN and 75% 1 mM aqueous buffer, were infused at a flow rate of 5 μL/min with a Harvard Apparatus (Hollister, USA) syringe pump.
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Ibotenic acid (10 μg/μl in phosphate buffer) was infused using a Hamilton micro-syringe at selected ICMS sites of the hand area in M1 unilaterally, as previously reported in detail (Liu and Rouiller 1999).
After the 2 h of reperfusion, 40 ml of 1% (w/v) solution of triphenyl tetrazolium chloride (TTC) in phosphate buffer was infused into aortic cannula, and the heart samples were stored at -70°C for subsequent analysis.
Solutions containing treatments were infused at 500 mL/h for 22 h/d and provided ∼3.3 Mcal/d.
Proteins were infused at 5 μL/min.
Solutions were infused at a rate of 20 ml/kg/hour for 2 hours.
Substances were infused at a slow rate to avoid trauma and vesicoureteral reflux (18).
The perfusion buffer at 37°C was infused at a rate of 0.1 mL/minute via the arterial cannula and removed from the venous cannula using a Minipulse peristaltic pump (Anachem, Luton, United Kingdom).
The left lung was infused at a pressure of 30 cmH2O with 10% buffered formalin, embedded in paraffin, sectioned at 4 μm thickness, and stained with hematoxylin and eosin.
Drugs are infused at a constant rate (1 ml/min) with an infusion pump.
Mean rumen ammonia concentrations increased linearly from 29.1 mg/l when no ERDP was infused to 184.5 mg/l when ERDP was infused at the highest level.
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