Sentence examples for buffer were incubated in from inspiring English sources
Ten microliters of each size-exclusion resulting fraction (in 0.1% sarkosyl TNT extraction buffer) were incubated in 1× loading buffer (containing only orange G (Sigma); 20% glycerol; TNT extraction buffer) and separated on a 2.5% agarose gel in a Tris-Glycine (1.45 g/L Tris-base (BioRad) ; 7.2 g/L Glycine (BioRad)) running buffer.
As described in Materials and Methods, 0.05 mg/mL of CFP-10 dissolved in the PBS buffer were incubated in the silicon gasket well (3 mm diameter) placed on the NPS surface.
NRC-03 and NRC-07 reconstituted in 50 m M TEAB buffer were incubated in the presence or absence of trypsin (2 μg) overnight at 37°C, dried, reconstituted in 0.1% TFA, and diluted 1 1 in Matrix Solution.
Dilutions of AmPDH and variant H103Y in 65 mM sodium phosphate buffer were incubated in an Eppendorf tube at different temperatures and the remaining enzymatic activity was determined regularly over 35 weeks (4 °C, 21 °C, 30 °C) and 24 h (40 °C and 50 °C) in duplicates.
Then, 100 μl binding buffer containing 10 kBq of In-DOTA-E-[c RGDfK ]2 and approprIn-DOTA-E-[c RGDfK ]20−6–8 × 10−11 M) of Ga(In-DOTA-E-[c RGDfK ]2lled In-DOTA-E-[c RGDfK ]2-E-[c(RGDfK)]2 and DOTappropriatefK)]2}2 in bindilutionser were incubated in the wells at 37°C for 1 h.
In general, DNA-binding experiments were carried out in the following manner: 5 μL NF-D1 (consisting of NF-01F and NF-14D) and 5 μL protein in binding buffer were incubated in a 96-well (half-area) microplate for 30 min at room temperature.
The 5.3 kb ssDNA (0.5 pM) in the blocking buffer was incubated in the flow chamber for 10 min and unattached DNA was removed by extensive washing as described for the FRET studies.
HPLC-purified 125I-Aβ40, 125I-Aβ40 H13G, 125I-Aβ40 H13R, 125I-Aβ40 H13,14G or buffer was incubated in vitro with unfixed cryosections (15 µm) of brain from a 20-month old APP, PS1 AD transgenic mouse using the same procedure used previously [33].
The purified p03 or p01 or the control buffer was incubated in a 20-μl reaction mixture containing 5 μCi of [γ-P]ATP (specific activity 3000 Ci/mmol, GE Healthcare) at 37 °C in ATPase buffer (50 mM Tris-HCl, pH 7.5, 0.1 M NaCl, and 5 mM MgCl2) for 30 min.
The integration assay was performed as follows: Post-nuclear supernatant (800 µg total protein) or the mitochondria-enriched fraction P1 (150 µg total protein) in cell fractionation buffer were incubated with in vitro-synthesized proteins for 60 minutes at 4°C.
