Sentence examples for buffer were incubated at from inspiring English sources
2 mL of the homogenate was then centrifuged in a micro-centrifuge for 5 min at 3000 g and 100 μl of the diluted supernatant (1 50 with incubation buffer) were incubated at room temperature onto a microtiter plate coated with a monoclonal capture antibody highly specific to the calprotectin heterodimeric and polymeric complexes.
2 mL of the homogenate was then centrifuged in a microcentrifuge for 5 min at 3000 g and 100 μl of the diluted supernatant (1 50 with incubation buffer) were incubated at room temperature onto a microtiter plate coated with a monoclonal capture antibody highly specific to the calprotectin heterodimeric and polymeric complexes.
Briefly, 3 ml of the reaction mixture containing 10 mM sodium nitroprusside and the seaweed extract (100 μg/ml) in phosphate buffer were incubated at 25°C for 150 min. After incubation, 0.5 ml of the reaction mixture was mixed with 1 ml of sulfanilic acid reagent (0.33% in 20% glacial acetic acid) and allowed to stand for 5 min for complete diazotization.
ABTS and 0.1 M citrate phosphate buffer were incubated at the optimum temperature of each protein 2 min before addition of enzyme.
Diluted cell lysate (1 : 200) and purified rSK (1 : 100) in renaturation buffer were incubated at 37°C for 1 h and 4°C for 6 h.
Membrane suspensions (50 μl) in 0.5 ml HEPES buffer were incubated at 4 °C with 0.05 2 nM [H]-granisetron (for 1 h) or [H]-palonosetron (for 24 h).
The homogenate was centrifuged in a micro-centrifuge for 5 minutes at 3,000 g, and 100 μl diluted supernatant (1 50 with incubation buffer) was incubated at room temperature in a microtiter plate coated with KIAA1199 monoclonal antibody (sc-164775, Santa Cruz, CA, USA).
A mixture of AcpM, 1 mM β-mercaptoethanol, and the buffer was incubated at 37°C for 30 min to ensure complete reduction of AcpM, and then the remaining components (except mtFabH) were added.
The secondary antibody (1 5000 in blocking buffer) was incubated at 25°C for 1 h before standard enhanced chemiluminescence detection.
