Sentence examples for buffer were immunoprecipitated with from inspiring English sources
For analysis of ubiquitination of fractionationed samples, equal volumes of S and P (resuspended in same as initial volume of lysis buffer) were immunoprecipitated with α-tubulin Ab and immunoblotted with ubiquitin Ab.
Whole cell lysates from EOL-1 cells were prepared utilizing RIPA buffer and were immunoprecipitated with an anti-EZH2 (Cell Signaling Technology, Beverly, MA, USA) antibody and protein G Sepharose (Pierce, Rockford, IL, USA).
Next, 1 mg of the extracted protein in the lysis buffer was immunoprecipitated overnight with anti-MLKL or anti-RIP1 antibody at 4 °C and then with protein A+G agarose beads (Beyotime) for another 4 h.
Cells were lysed in NP-40 buffer, then lysates were immunoprecipitated with specific antibodies and examined by immunoblotting.
For analysis of endogenous RelA ubiquitination, total cellular lysates, prepared with lysis buffer as above, were immunoprecipitated with anti-RelA antibody and analysed by immunoblotting with anti-ubiquitin antibody.
Cells were lysed in lysis buffer and the clarified lysates were immunoprecipitated with the appropriate antibodies.
Lysates from the different strains were incubated in a reaction buffer containing KTPγS, and the putative targets were immunoprecipitated with the Ty-tag.
About 6 × 106 cells were treated with 1% formaldehyde and sonicated in 600 μl of protease inhibitor-containing buffer, and then the chromatin was immunoprecipitated with a rabbit anti-serum anti-acetyl histone H4 (Upstate Biotechnology) essentially according to the manufacturer's specifications.
The cell lysate was immunoprecipitated with the indicated antibodies.
Wash beads twice with 1 ml cold buffer and suspend protein A/G agarose in 50 ul RIPA or IPP150 buffer per sample to be immunoprecipitated.
