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Briefly, cells suspended in extraction buffer were homogenized in a Dounce homogenizer using 15 strokes.
The tissue and 10 vol. of RIPA buffer were homogenized in a 2 ml glass homogenization tube with Teflon-coated homogenizer (20 up-and-down motions).
For ATP analysis, pooled larvae (10 larvae/200 μl buffer) were homogenized in 1 × Reporter lysis buffer (Promega, Madison, WI, USA), and immediately flash frozen.
Samples of liver and kidneys (100 mg mL-1 buffer) were homogenized in 50 mM phosphate buffer (pH 7.0), and then centrifuged at 10,000 rpm for 15 min; the supernatant thus obtained was used for biochemical analysis.
To measure glycosaminoglycan (GAG) and DNA content in the NP and AF, samples in Ambion® KDalert™ lysis buffer solution were homogenized in a tube rotator O/N at 4 °C, whereas samples in Complete Lysis-M EDTA-free buffer were homogenized in a TissueLyser II (Qiagen) for 2 × 30 s at 20 Hz.
Similar(55)
The brain tissue in the lysis buffer was homogenized in a Potter homogenizer (cells were not needed for this step).
The pools were homogenized in lysis buffer (Buffer AVL; QIAGEN) by using sterile disposable tissue grinders, and RNA was extracted.
The samples were homogenized in Buffer RLT using a QIAshredder homogenizer.
Tissues were homogenized in buffer containing 80 mM PIPES (pH 6.8), 1 mM MgCl2, 2 mM EGTA.
In brief, fresh tissues were homogenized in buffer containing protease inhibitors and centrifuged at 14000 rpm at 4°C for one hour.
Rat livers were homogenized in buffer A, spun at 800×g for 5 min, the supernatant collected and Triton X-100 was added to 1% final.
More suggestions(15)
buffer were tested in
buffer were solubilized in
buffer were spotted in
buffer were fixed in
buffer were lysed in
buffer were heated in
buffer were suspended in
buffer were retained in
buffer were resuspended in
buffer were included in
buffer were printed in
buffer were dissolved in
buffer were collected in
buffer were resolved in
buffer were frozen in
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