Sentence examples for buffer were heated in from inspiring English sources

For antigen retrieval, slides in citrate buffer were heated in a microwave for 20 minutes and allowed to cool before blocking with 10% normal goat serum.

Aliquots of 50 micrograms in Laemmli buffer were heated in boiling water for 5 min, electrophoresed on 10% polyacrylamide gels, and transferred to PVDF membranes (Biorad, Hercules, CA).

Slides immersed in buffer were heated in the microwave at full power until full pressure was achieved (5 min) then heated for an additional 7 min at 20%% power.

In the electrophoretic mobility shift assay (EMSA) 51 the Cy5 tagged AraHH001 aptamer in 1× selection buffer was heated in 80°C for 10 min to permit secondary structures to form, and 10 nmol/L of Cy5 tagged AraHH001 was then incubated with a different concentration of cTnT on ice in 4°C for 30 min.

Proteins in sample buffer were heated at 37°C, resolved by SDS-polyacrylamide gel electrophoresis in 10% acrylamide gels using tricine buffer, and transferred to nitrocellulose membranes.

Mouse or human protein samples in SDS sample buffer were heated to 95 °C for 10 min and separated on SDS-polyacrylamide gels.

Samples in SDS Loading Buffer were heated (100°C, 5 min), subjected to SDS-PAGE, transferred to PVDF or nitrocellulose membranes, and blocked (4°C, overnight) in PBST (PBS with 0.05% Tween 20) containing 5% non-fat dry milk or 5% BSA.

Slides in citrate buffer (pH 6.0) were heated in the steamer for 30 min.

For immunohistochemistry, sections in 10 mM sodium citrate buffer (pH 6.0) were heated in a microwave oven and kept at 95°C for 10 minutes.

Beads were washed with RIPA buffer for three times and were heated in SDS loading buffer.

The sample including 0.9 mL homogenate and 0.1 mL of reaction buffer was heated to 37°C in a water bath for 15 min, and then the enzymatic activity was determined by measuring the changes in absorbance at 460 nm using a 96-well plate reader and expressed as U/g weight.