Sentence examples for buffer were heated at from inspiring English sources

Proteins in sample buffer were heated at 37°C, resolved by SDS-polyacrylamide gel electrophoresis in 10% acrylamide gels using tricine buffer, and transferred to nitrocellulose membranes.

Samples (50 µg protein in 30-µl Laemmli buffer) were heated at 95°C for 5 min, resolved by 10% SDS-PAGE (50 µg/lane), and electro-transferred to PVDF.

Cell lysates in SDS-PAGE sample buffer were heated at 95°C for 5 min, separated by 12.5% SDS-PAGE, and transferred to a nitrocellulose membrane.

The PCR conditions were as follows: 1 cycle at 94°C for 5 min; 35 cycles at 94°C for 30 s, 60°C for 30 s, 72°C for 1 min, and 1 cycle at 72°C for 10 min. PCR products mixed with loading buffer were heated at 95°C for 5 min and quickly chilled on ice.

20 μl of pre hybridization buffer was heated at 98°C for 2 min, cooled to 42°C, applied to the cDNA-array and sealed with cover slip.

Five μL of TFO RNA mixed with 5  μL of urea loading buffer was heated at 92°C for 2 minutes and immediately chilled on ice.

Slides immersed in buffer were heated in the microwave at full power until full pressure was achieved (5 min) then heated for an additional 7 min at 20%% power.

After suspending 50 µg of cell extract in SDS sample buffer, proteins were heated at 95°C for 5 min and separated by 4 12% SDS-polyacrylamide gel electrophoresis.

After addition of Laemmli buffer, samples were heated at 100°C for 10 min and separated by SDS-PAGE on 12% Criterion™ XT Bis-Tris Precast Gels (Bio-Rad) followed by silver staining.

After adding 50 μl of SDS sample buffer, the samples were heated at 95°C for five minutes, and centrifuged for one minute at 13,000 × g.

For antigen retrieval using citric acid buffer, the slides were heated at 120°C for 20 min and then cooled for 20 min at room temperature.