Sentence examples for buffer were ground with from inspiring English sources

Between 50 and 100 mg of embryogenic tissue or embryos in extraction buffer, were ground with a pestle in 1.5-ml Eppendorf tubes.

Samples were ground with extraction buffer, added at a ratio of 25 : 1 for leaves and 62.5 : 1 for lyophilised tissue and consisting of PBS at pH 7.4, 10 mM Na2SO3, 2% (w/v) PVP40000, and 0.2% (w/v) BSA and supplemented with 0.05% (v/v) Triton X-100.

Protein extracts for Western blot analyses were prepared as follows: five dehulled seeds were ground with 1 mL of extraction buffer (500 mM NaCl in 50 mM Tris-HCl buffer, pH 7.0) using mortar and pestle.

Lyophilized A. machipongonensis cells were ground with a mortar and pestle and suspended in 10 mM Tris Cl buffer (pH 8.0), containing 2% sodium dodecyl sulfate (SDS), 4% 2-mercaptoethanol, and 2 mM MgCl2.

Dried samples were ground with a grinding mill and sieved through a 1-mm sieve.

Samples were ground with a laboratory mill (Bamix, Mettlen, Switzerland).

The sections were ground with a grinding machine (Planopol-V, Struers, Rødovre, Denmark), to an appropriate thickness of 30 40 μm.

If tissue was ground with a FastPrep, homogenize with the cold buffer for an additional 40 s.

For enzyme assay, 0.3 g of leaf was ground with 3 mL of ice-cold 50 mM sodium phosphate buffer (pH 7.5).

Tissue was ground with mortars and pestles in liquid nitrogen.

Seed was ground with liquid nitrogen by mortar and pestle.