Sentence examples for buffer were fractionated by from inspiring English sources

Samples containing 50 75 µg of protein in reducing sample buffer were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis.

The whole cell lysates and immunoprecipitated ABCG1 eluted from the beads by 1× SDS loading buffer were fractionated by 8% SDS-PAGE, which was followed by immunoblotting with specific antibodies indicated.

The NaOH was neutralized by the addition of 5 µL of 4 M acetic acid and after the addition of Laemmli buffer proteins were fractionated by SDS polyacrylamide gel electrophoresis and analyzed by immunoblotting using anti-HSF1 (Neef et al. 2010) and anti-PGK1 (22C5D8; Abcam) antibodies.

Cells were harvested at indicated time points, lysed in reducing Laemmli buffer and proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to standard procedures [33].

For in-gel analyses, reaction products from in vitro ubiquitin conjugation assays, halted by the addition of SDS sample buffer containing reducing agent, were fractionated by SDS/PAGE (Novex NuPAGE 10% Bis-Tris gel; Life Technologies) using Mes SDS running buffer.

The polymers were fractionated by fractional precipitation.

The LC program included a 5-min linear gradient from buffer A (50% acetonitrile with 0.1% formic acid) to buffer B (99.9% acetonitrile with 0.1% formic acid), a constant elution in buffer B for 10 min, followed by a linear gradient return to buffer A in 5 min. Samples were fractionated by a 2.1×50 mm Eclipse Plus-C18 column (Agilent) with a flow rate of 0.5 ml min−1.

Whole protein lysates (40 to 50 μg protein) were fractionated by Tris-glycine buffered 10% SDS-PAGE, transferred to Polyvinylidene difluoride membrane (Roche) and probed with antibodies to prokineticin receptor 2 (PROKR2) (Santa Cruz, Santa Cruz, CA, USA) and β-actin (Sigma).

Whole cell lysates (40 to 50 μg of protein) were fractionated by Tris-glycine buffered 10% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membrane (Hybond-P, Amersham Biosciences, Little Chalfont, UK).

The reaction, stopped by the addition of Laemmli sample buffer, was fractionated on 10% SDS-PAGE and subsequently silver stained as described [39].

Gradients were fractionated by a Piston Gradient Fractionator (BioComp).