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Larva dissected in HL3 buffer were fixed in 4% formaldehyde, washed, washed in PBS Tween 0.1%, pre-hybridized for 2 h at 55°C, and hybridized overnight with 2 mg of probe.
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After washing twice in FACS buffer cells were fixed in 300 µl 1% p-formaldehyde in PBS.
After 3 washes with FACS Buffer the cells were fixed in cold 2% Paraformaldehyde in PBS and 10,000 Thp-1 cells were acquired using a FACSCalibur flow cytometer.
Following incubation, the cells were washed three times with FACS buffer and cells were fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Inc., Warrington, PA).
After buffer removal, parasite trails were fixed in PBS containing 3.6% formaldehyde, blocked and stained with anti-T.
178 cervical cancer biopsies were fixed in formalin buffer and embedded in paraffin.
The specimens were fixed in 10% buffered formalin for 1 to 2 weeks.
The remaining snails were fixed in 10% buffered formalin.
Bone and spleen were fixed in 10% buffered paraformaldehyde.
Lung samples were fixed in 10% buffered formalin.
All nephrectomy specimens were fixed in 10% buffered formalin.
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