Sentence examples for buffer were estimated by from inspiring English sources
ATPase activities on purified protein (1 µg in 30 µl buffer) were estimated by the malachite green method as described [31] using LmrCD in 0.05% (w/v) DDM or LmrA in 0.1% (w/v) fos-choline-12.
Salivary mutans streptococci, lactobacilli and buffer capacity were estimated by selective chair-side kits (Dentocult® SM-Strip mutans, Dentocult® LB and Dentobuff® Strip; Orion Diagnostica, Espoo, Finland) according to the manual of the manufacturer.
Bound protein was eluted with 50 mM Tris, 500 mM NaCl, 0.5 M imidazole, and dialyzed into buffer D. Protein concentrations were estimated by comparing protein preparations to serial dilutions of a bovine serum albumin (BSA) standard in SDS-PAGE gels stained with Coomassie Brilliant Blue R (Sigma).
Samples, dissolved in methanol, were analysed using a C18 column (RP C18, Xterra, 5 μm, 4.6 × 150 mm) and a mobile phase consisting of MeOH and phosphate buffer (0.1 M, pH 7.5) compounds were estimated by a comparison of its retention time to that of standards of known log P values.
Convection rates were estimated by the increases in buffer solution temperature.
Total cell protein was extracted by RIPA buffer (Pierce) after 60 h and AhR, αB-crystallin and β-actin protein levels were estimated by immunoblotting.
The binding constants were estimated by the change of migration time of the analytes in the buffer solution containing different concentrations of SARS 3CL proteinase.
The enzyme stability at different pHs were estimated by measuring the residual activity after incubating the enzyme solution in buffers at pH 3.0 10.0 at 25°C for 1 h.
