Sentence examples for buffer were dissolved in from inspiring English sources
Rat tissue extracts (2.5 mg, prepared in IEF lysis buffer) were dissolved in a final IEF buffer volume of 2.5 ml, applied to MicroRotofor (Bio-Rad), and electrophoresed for 1.5 2.5 hours (1W constant power, 4°C).
Cell pellets washed with 10 ml MACS buffer were dissolved in 500 μl of MACS buffer.
The first part of the above protein pellets (1 mg RJ/100 μl buffer) was dissolved in 40 mM of (NH4 HCO3 (Sigma).
The Apollo reaction buffer liquid, catalyst, fluorescent dyes and buffer additives were dissolved in deionized water, and shaken to make the Apollo® 567 staining reaction solution.
RIPA buffer-insoluble pellets were dissolved in urea buffer (7 M urea, 2 M thiourea, 4% CHAPS, 30 mM Tris-HCl, pH 8.5) and sonicated.
After washing the cells with HBSS buffer, the formazan crystals were dissolved in DMSO for 30 min on a shaker.
After sedimentation, the pellets were dissolved in buffer containing 2% SDS.
Precipitated proteins were dissolved in buffer and dialyzed against distilled water and lyophilized [ 34].
The prodrugs were dissolved in buffer, (DMSO was also added in case of poor aqueous solubility), incubated, and analysed hourly until total decomposition was observed.
Without further purification, all Y-DNAs were dissolved in buffer to give a final concentration of 10 μM.
