Sentence examples for buffer were denatured at from inspiring English sources

DIG-labelled RNA probes (100 200 ng/ml diluted in hybridisation buffer) were denatured at 80°C for 5 minutes then cooled on ice.

Samples of EF-Tu preparations (250  μL, 37  μM) and controls (dialysis buffer) were denatured at 90°C for 10 min.

F.S. cDNA-RNA duplexes in hybridization buffer were denatured at 98° for 3 minutes and then allowed to hybridize at 70°C for 5 hours.

Samples in buffer were denatured at 95°C for 10 minutes, incubated at 40°C for 5 minutes, collected by centrifugation then applied to arrays, which were incubated for 16 hours at 40°C at 60 rpm.

Samples in Laemmli buffer were denatured 5 min at 95°C, plunged on ice for 2 min, loaded into a 4 20% SDS-PAGE (#456-1093; Biorad, Hercules, CA) for separation in 1× Tris Glycine SDS Running Buffer (#161-0732; Biorand, and transferred to Whatman nitrocellulose membrane (#10439396; Sigma-Aldrich) in Tris Glycine transfer buffer (#351-087-131; Quality Biological) diluted in 20% ethanol.

After washing with lysis buffer, the beads were denatured at 95 °C in NuPAGE buffer (Invitrogen) and run on SDS-PAGE, followed by immunoblotting.

Spy variants were diluted to 6.5 μM in 10 mM sodium phosphate buffer (degassed), pH7.5 and were denatured at a heating rate of 2°C/min from 10°C to 80°C in a 1 mm path length quartz cuvette.

Protein in loading buffer was denatured by heating at 100°C for 10 minutes and then separated on a SDS-polyacrylamide gel by electrophoresis.

After three washes in lysis buffer, an equal volume of 2× SDS-PAGE sample buffer was added and samples were denatured at 100 °C for 5 min.

Protein samples dissolved in the sample buffer and the marker protein were denatured at 100 °C for 5 min before being loaded onto the gel.

The Hy3TM-labeled sample (25 μL) with 25 μL of hybridization buffer was denatured for 2 min at 95°C, incubated on ice for 2 min, and then hybridized to the miRCURYTM LNA Array (v.16.0) (Exiqon), according to the manufacturer's instructions, for 16 20 h at 56°C in a 12-Bay hybridization system (Hybridization System; Nimblegen Systems, Inc., Madison, WI, USA).