Sentence examples for buffer were combined with from inspiring English sources

Phage stocks in either in LB or TM buffer were combined with the cells on ice for 15 minutes at the multiplicity of infection of approximately 10.

Cell suspensions in digestion buffer were combined with 4U of Zymolyase (Zymo Research), incubated at 37°C for 4 hours, and then stored at 4°C until ready for use.

Thereafter, the wells were washed with PBS by cautious pipetting and the recovered buffer was combined with the GM previously recovered from the wells.

Thereafter, the lysed samples were removed from wells by vigorous pipetting, the wells were washed with PBS and the recovered buffer was combined with the lysate previously recovered from the wells.

Briefly, 400 μL of blood lysate (200 μL whole blood + 200 μL RNA isolation buffer) was combined with an equal volume of acid phenol, vortexed, and centrifuged at 13200 g for 15 min. One half of the aqueous phase was transferred to a fresh tube and vortexed with 1.25 × volume of 100% ethanol.

To avoid starch interference and improve the RNA yield, 1 vol of Qiagen RLT extraction buffer was combined with an equal volume of the following solution: 2 % (w/v) CTAB (Cetyl Trimethyl Ammonium Bromide); 2% (w/v) PVP 40; EDTA 25 mM; Tris/HCl 100 mM (pH 8).

Briefly, RIA buffer [80% phosphate-buffered saline (PBS), 20% DMEM, 2% normal rabbit serum], 100 μL cold standard (rat PRL-RP-3) or unknown sample, rPRL-s-9 antiserum (final dilution of 1 437,500 in RIA buffer), and [I]-rat-PRL (PerkinElmer, Wellesley, MA, USA; using 15,000 counts per tube diluted in RIA buffer) were combined and incubated with shaking, overnight at 4°C.

Briefly, 20 μL protease and 200 μL buffer AL were combined with 200 μL whole blood and incubated at 56°C for 10 min. After incubation, 200 μL ethanol was added, and the entire mixture was applied to a QIAamp spin column.

30  μL of a solution containing SDS loading buffer and mercaptoethanol were combined with 50  μg of sample protein and lysis buffer up to 100  μL.

At time zero, 2x stocks of pre-warmed enzyme or buffer-only control were combined with Ub-AMC to yield 500 nM GST-tagged enzyme and 500 nM Ub-AMC in a final reaction volume of 100 µl.

EMSAs were performed as follows: P-labeled DNA (~0.5 ng, 50,000 cpm) and 2 μg of nuclear protein were combined with buffer (final concentrations: 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM sodium chloride, 0.5 mM magnesium chloride, 1 mM DTT, 20 μg BSA, 10% glycerol, 0.5% Triton X-100) and poly dI-dC (1 μg) in a final volume of 20 μl.