Sentence examples for buffer were cleared with from inspiring English sources

Whole-cell lysates in lysis buffer were cleared with 1.0 μg nonimmune rabbit IgG (Santa Cruz) together with 30 μl of protein A-Sepharose beads (Pierce).

After lysis with Reporter Lysis Buffer (Promega), lysates were cleared with centrifugation for 15 min at 14,000 rpm and cell extracts were incubated with the luciferase substrate reagent for 30 min at room temperature.

When a decoder ℱ m finishes decoding the codeword in its buffer, the buffer is cleared and updated with a new codeword from a long input buffer according to some scheduling scheme.

Once you save the file, the information is copied to the hard disk and the buffer is cleared.

If a control phrase is recognized in the buffer, the whole buffer is cleared and an operational action is performed.

Leaves were cleared with 100% ethanol, re-hydrated and stained with aniline blue (0.05% in phosphate buffer pH8.0) for 24 h.

The aorta was cannulated for Langendorff perfusion, and the coronary arteries were subsequently perfused with warmed (37°C) 150 μM EGTA digestion buffer until they were cleared of blood.

Homogenates were cleared by dilution with 2 vol of RIPA buffer 2 (RIPA buffer 1 without NaCl).

Cell lysates were cleared by centrifugation and mixed with an equal volume of 2 × Laemmli sample buffer and incubated at 95 °C for 5 min.

For purification of hTpx2 lysates were cleared and diluted 1 3 (to 50 mM NaCl) with 50 mM sodium phosphate buffer pH 7.5 as done for hKif15.

Aliquots of 500 μg of total protein in equal amount of lysis buffer were pre-cleared with protein A beads (Cell Signal Technology) for 2 h.