Sentence examples for buffer were centrifuged at from inspiring English sources

Harvested cells suspended in Tris buffer were centrifuged at 17,000 g for 8 min at 4°C.

The cells suspended in the buffer were centrifuged at 10 000  g for 10 min at 4°C, and then the supernatant (containing the total protein fraction) was carefully transferred to a new tube.

For whole-cell proteins, approximately 50 mg mL−1 of cells or spheres (wet weight) was lysed by boiling in SDS sample buffer (Laemmli, 1970) for 10 min. For examination of released proteins, spheres that had been prepared by treatment with TL buffer were centrifuged at 10 000 g for 20 min. Supernatants were taken off and filtered through 0.22-μm sterile filters to remove any residual spheres.

Briefly, 200 µl of buffer CER I supplemented with protease inhibitors were added to the homogenate and then sonicated on ice for 20 seconds and then incubated on ice for a further 10 min. The homogenate with 10 µl of CER II buffer was centrifuged at 12,000×g for 10 min at 4°C.

After thawing, 0.5 ml plaque samples in TE buffer were centrifuged for 5 min at 13,000× g washed and vortexed with 200 μl TE.

After four washes in TNES buffer, samples were centrifuged at 3000 × g for 2 min and resuspended in 20 ml SDS-sample buffer (0.5M Tris HCl, pH6.8, 20% glycerol, 2% SDS, 5% 2-mercaptoethanol, 4% bromophenol blue).

Combined supernatants (homogenate) in buffer A were centrifuged at 12,000 g for 15 min.

After washing the beads in 1 × IP buffer, they were centrifuged at 6000 r.p.m. (=3800 × g) for 2 min at 4 °C, and the supernatant was discarded.

Cells were harvested after 48 hours of transfection using RIPA lysis buffer and were centrifuged at 5000  g for 15 min to pellet cell debris.

Cervical brush samples collected in Phosphate Saline Buffer (PBS) were centrifuged at low speed and the cell pellets underwent DNA extraction using QIAampTissue kit (Qiagen, Milan-Italy).

Solubilized cell lysate, combined NEF/LSP fractions, or partially purified proteins in buffer B were centrifuged at 105000 g for 30 min at 4 °C to remove any particulate matter.