Sentence examples for buffer were boiled for from inspiring English sources
Twenty-four hours after transfection, cells were lysed in RIPA buffer, containing protease inhibitor cocktail at 4°C for 30 min, spun down at 12,000 rpm for 10 min. Protein extracts in 40 μL of 2× SDS-PAGE sample buffer were boiled for 10 min. Samples were separated on SDS-PAGE gel and transferred to NC membrane.
Subsequently, two volumes of each skeletal muscle homogenate and one volume of SDS-sample buffer were boiled for 4 min. Next, 13% polyacrylamide gels containing 0.1% SDS were loaded with equal amounts of protein from each sample, and electrophoresis was performed using a Mini-Protean 3 Electrophoresis Cell Bio-Rad Laboratoriess, Hercules, CA).
Pull-down supernatants and pellets with loading buffer were boiled for 10 min.
30 μg of cell lysates (1 % Nonidet buffer) were boiled for 10 min at 60 °C in Laemmli sample buffer.
Protein G-precipitated protein complex was recovered by centrifugation and harvested beads resuspended in 30 μl of 2 × SDS PAGE sample buffer were boiled for 5 min.
Protein (50 μg) in 2× Laemmli SDS sample buffer were boiled for 5 min and after centrifugation loaded onto a SDS-PAGE gel.
Finally, the mixture of beads and SDS loading buffer was boiled for 10 min, and the supernatant was collected.
Fractions added with SDS-PAGE loading buffer were boiled at 100 °C for 10 min, then centrifuged (4 °C, 12,000g, 10 min) before loading on gels (TGX Stain-Free™ FastCast™ Acrylamide Kit, 12%, Bio-Rad, Cat. No. 161-0185) for SDS-PAGE.
Equal amounts (75 µg) of cytoplasmic or nuclear homogenate in 4× Laemlli buffer were boiled at 95°C for 5 minutes then loaded in the wells of at 7.5% gel.
Following the addition of sample buffer, samples were boiled for 3 min and separated by SDS-PAGE (8% acrylamide).
