Sentence examples for buffer were applied for from inspiring English sources

After extensive wash the secondary antibodies (in blocking buffer) were applied for 1.5 hours at RT in darkness.

Either rat anti-mouse CD31 or isotypic antibodies 1∶100 in 10× diluted blocking buffer were applied for 1.5 h and then sections were washed with PBS.

Phalloidin-Alexa Fluor 568 (Invitrogen A12380, diluted 1 50 in blocking buffer) and/or BrdU-FITC (Becton-Dickinson 347583, diluted 1 4 in blocking buffer) were applied for 30 min before final washes and mounting in Vector-shield containing 0.5 mg/ml DAPI (Sigma D9542).

Renatured mda-7/IL-24 protein was achieved by dialysis (the concentration of mda-7/IL-24 was 0.10 mg/mL at the beginning of dialysis) with the decrease of urea concentrations (6, 3, 1.5, 0.75, and 0 M), where each dialysis buffer was applied for 12 h at 4°C with mild agitation.

After cooling, monoclonal mouse anti-human CD68 clone PGM1 (DAKO, Denmark, 1∶25) in 1X wash buffer was applied for 45 minutes (Dako) at room temperature.

A 1 : 100 dilution of α-LmjMCA antibody in TB buffer was applied for 1 h at room temperature.

However, the higher pH (5.5 8.0) of the incubation buffer was applied for phosphoprotein enrichment since the lower pH (that is used for phosphopeptide enrichment) caused protein precipitation (Machida et al. 2007).

Primary antibodies in blocking buffer were applied to the cells for 1 hr at RT, followed by three 5 min washes in PBS.

Synthetic NLP-24 peptides (all synthesized with amidated C-termini), opioid agonists, and blockers diluted in HBSS (Hank's Balanced Salt Solution) buffer were applied to the cell for 10 s, and fluorescence at 530 nm was monitored (excitation wavelength 470 nm) with a Zeiss Axio Observer A1 microscope and ZEN software.

Briefly, sera diluted 1 101 in the sample buffer were applied to the wells and incubated for 30 min.

Briefly, diluted CSF and plasma samples (1∶2000 and 1∶400, respectively in dilution buffer) were applied to antibody pre-coated ELISA plates for 30 min with gentle agitation.