Sentence examples for buffer were annealed at from inspiring English sources

Prior to any interaction all RNAs in buffer were annealed at 90°C for 3 min, cooled to room temperature for 30 min. For all reactions 8 µl aliquots were loaded after diluting with loading buffer (10% (w/v) sucrose, xylene cyanol, bromophenol blue) under a power of 5 W on native 5 8% polyacrylamide (37∶1) gel in 1× TBE.

isRNAs were annealed at a concentration of 50 μM in a buffer containing 30 mM HEPES-KOH (pH 7.4), 100 mM sodium acetate, and 2 mM magnesium acetate.

Primers were annealed at 68°C for 35 cycles.

gyrA amplifications were annealed at 58°C and parC reactions were annealed at 52°C.

Primers were annealed at 60°C over 45 cycles.

Primers amplifying transcripts 1 and 2 were annealed at 35°C, primers amplifying transcripts 3 and 4 were annealed at 40°C.

After the synthesis of the sol-gel, cleaned GaN substrate was spin coated with the prepared sol-gel three to five times for the deposition of a thin NiO buffer layer; consequently, the substrate was annealed at 180°C for 20 min. After the annealing, the sample was left in the preheated oven for 4 h at 450°C in order to have a pure phase of NiO.

The third condition involved adding assay buffer (pH 7.5) to primer−template DNA that was annealed at pH 10 and incubating the sample for approximately five half-lives of the N-OPdG ring closure (2 h) before initiating polymerase catalysis.

Before the Ge-Sn film started growing, the Si substrate was annealed at 1000°C, and the buffer Si layer was grown at 700°C.

Oligonucleotides were annealed in buffer containing 20 mM Tris-HCl (pH 7.8), 100 mM sodium acetate, and 5 mM magnesium chloride in a 1 1 ratio at 65 °C for 20 min followed by slow cooling.

RNA solutions of 200 nM were annealed in sodium phosphate buffer by heating at 95 °C for 1.5 min followed by flash cooling.