Sentence examples for buffer were analyzed and from inspiring English sources

For Western Blot, 12 µl of a sample containing 5 fly heads homogenized in 30 µl of a classical loading buffer were analyzed and detection was performed by classical enhanced chemiluminescence (ECL™, GE Healthcare) using an antibody against GFP (Biovision, Mountain View), Rh1 (4C5 ascites, DSHB, Iowa), β-tubulin (E7, DSHB, Iowa) or the Drosophila glutamate receptor (monoclonal 7G11 [45]).

Cells diluted in binding buffer were analyzed by FACScan (BD Biosciences) and quantified by CellQuest software (BD Biosciences).

Supernatant and bound proteins extracted with sample buffer were analyzed for gelatinase activity by zymography.

Supernatant and bound proteins extracted with sample buffer were analyzed for gelatinase activity using zymography.

TE buffer was analyzed as blank.

For each analysis, the same amount of TE buffer was analyzed as the blank.

The stability of the protein in different pH buffer systems was analyzed, and it was found that >70%% endoglucanase and >80%% β-glucosidase residual activities were retained after 60 min incubation period in pH 2.0 to pH 11.0 (Fig. 5c, d), except where no significant loss of endoglucanase and β-glucosidase activity was observed at pH 5.0 and pH 7.0, respectively.

Performance of the proposed output-queued shared-buffer switch is analyzed and compared.

After fixation in 1% paraformaldehyde for 10 min at 25°C, cells were washed in PBS and permeabilized for 10 min at room temperature in saponin buffer (0.5% (weight/volume) saponin, 5% FBS and 10 mM HEPES, pH 7.4, in PBS) Permeabilized cells were incubated for 45 min with phycoerythrin-conjugated Ab to TCR in saponin buffer, were washed in saponin buffer and were analyzed on a FACS Calibur.

Beads were then washed with NETN buffer three times, and samples were boiled with 2× SDS loading buffer, and were analyzed by immunoblot with indicated antibodies.

Cells were washed twice in Staining Buffer and were analyzed using LSR 1 or LSRII (BD Bioscience) in the Stanford Shared FACS Facility.