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Cells lysed in RIPA buffer were analysed by HA and SUMO-1 immunoblot but this did not reveal the presence of the fusion proteins within cells.

Stability of the compounds in aqueous buffers was analysed by reversed-phase HPLC with UV and ESI-MS detection (Supplementary Fig S1), and their solubility was checked using Millipore low-binding hydrophilic centrifugal filters and HPLC with UV detection.

After washing twice in 1x permeabilization buffer cells were analysed by flow cytometry as described above.

After washing with 1 ml of 1X permeabilization buffer, cells were analysed by flow cytometry as described above.

500 µl binding buffer containing 20 µl of a 50% slurry of glutathione sepharose were added and reactions were rotated at room temperature (RT) for 90 min. After washing three times with binding buffer, precipitates were analysed by SDS-PAGE and immunoblotting with the anti-Sam68 antibody C20 or the anti-GST antibody Z5 (both from Santa Cruz Biotechnology).

After having been washed three times with washing buffer, parasites were analysed by flow cytometry (FACSCalibur).

After washing, the beads with lysis buffer, immunocomplexes were analysed by immunoblot.

After adding annexin, buffer cells were analysed by a FACS Canto II flow cytometer (BD Biosciences).

After washing in Perm/Wash Buffer cells were analysed by FACScalibur flow cytometer using CellQuest software.

The bound proteins were liberated by boiling in Laemmli sample buffer and were analysed by SDS/PAGE.

After protein capture, beads were washed 4 × with Hepes lysis buffer and resuspended in gel sample buffer and proteins were analysed by SDS PAGE and immunoblotting.