Sentence examples for buffer were added into from inspiring English sources
For the internal control of β-gal activities, 90 µl of assay reagent (4 mg/ml ONPG, 0.5 M MgSO4, β-mercaptoethanol and 0.4 M sodium phosphate buffer) were added into each well.
After 30 min shaking at 4°C, viruses at a multiplicity of infection (MOI) of 500 VP/cell in 50 µl binding buffer were added into each tube, and they were shaken at 4°C for 1.5 hours.
Agarose-conjugated anti-p-Tyr (pY99) antibodies (Santa Cruz Biotechnology Inc., USA, CA) (prewashed three times with IP buffer) were added into each peptide mixture and incubated overnight at 4°C with gentle rotation.
DTT (1 mol/L 10 μl) of Caspase-3 detection kit and 50 μl 2 × reaction buffer were added into 10ul supernatant sample protein, and then 10 μl 1 mmol Caspase-3 substrate Ac-DEVD-pNA was added.
When the sample front moved upstream the viewing window and reached the pink control line labeled "A," 2 drops of chase buffer were added into the oval well at upper end of the kit.
Then, 40 60 μl of buffer A and 40 μl of T-cell suspension (∼1×10 2×10 cells/ml) in the same buffer were added into the small chamber.
Volumes were adjusted to 100 µL with carbonate-bicarbonate buffer and 150 μL of a reaction buffer was added into each well.
Then, 100 μL of streptavidin-HRP (diluted at 1 5000 in PTM blocking buffer) was added into each well and incubated for 1 h at RT, 600 rpm to detect antigen-antibody binding.
After 24 and 48 h, 20 μL of MTT solution (5 mg/mL in PBS buffer) was added into each well and incubated for another 4 h.
After 48 h, 20 μL of MTT solution (5 mg/mL in PBS buffer) was added into each well and incubated for another 4 h.
