Sentence examples for buffer were added for from inspiring English sources

Alexa Fluor-conjugated secondary antibodies, diluted 1 200 in blocking buffer, were added for 1 hour at room temperature.

After an incubation time of 10 min at room temperature, additional 400 μl of binding buffer were added for a final volume of 500 μl.

After three washes, anti-mouse or rabbit Europium-labeled secondary antibodies (1 μg/ml in assay buffer) were added for an additional hour.

Annexin V-FITC and PI (5  μl per 100  μl buffer) were added for 15 min at room temperature (RT) in darkness, and then analysed by flow cytometry (Becton Dickinson, Franklin Lakes, NJ, USA) within 1 h.

The frozen whole blood with anticoagulant was thawed in a 37°C water bath and 4 volumes of red blood cell lysis buffer were added for lysing red blood cells.

Wells were washed twice with SM buffer and either 220 μl of a phage stock (10 PFU/ml) or 220 μl of SM buffer were added for test and control purposes, respectively.

After PBS-tween/PBS wash Cyanine 5 (Cy5) directly conjugated to tyramide (Perkin-Elmer, Waltham, MA, USA) at 1 50 dilution in amplification buffer was added for 10 min for target antibody detection.

No buffer was added for pH stabilization.

The vial was heated and optionally buffer was added for pH-adjustment.

Seventy microliters of lysis buffer was added for every 20 µl of packed cell volume.

The homogenate was transferred into a precooled 40 ml tube, and 6 ml of extraction buffer was added for two times to clean the mortar.