Sentence examples for buffer were added at from inspiring English sources

Sigma RBC lysis buffer were added at room temperature.

GA3 and W5 buffer were added at 60 min after adding luciferase substrate (indicated with an open triangle).

The plant hormones and W5 buffer were added at 60 min after adding the luciferase substrate (indicated with an open triangle).

Purified Cx26-V5-His6 Cx26-V5-His6 Cx26-V5-His6uffer were added at 1:200 to themichannels.

Biotinylated anti-MRP8/14 (Abcam, Cambridge, UK), diluted 1/2000 in sample buffer, were added at a volume of 100 μl/well, and incubated at 4°C over night.

In order to prevent loading issues, a voltage buffer was added at the end which seemed to help draw a stronger signal.

The lysis buffer was added at a ratio of 20 µl per 1×105 cells.

400 μl of pre-chilled binding buffer was added at the end.

Plates were then washed three times with 300  μL/well washing buffer, and streptavidin-alkaline phosphatase (1 : 20000 in assay buffer) was added at 100  μL/well.

Alkaline phosphatase-conjugated goat anti-human IgM, IgA, or IgG (diluted 1 7,000 in the sample buffer) was added at 100 μL/well and incubated at 4°C overnight.

A confirmed VL tissue sample (AF61/2834/02) was homogenised using a pestle and mortar and 18 ml of NucliSens™ lysis buffer was added at RT.