Sentence examples for buffer wells were incubated from inspiring English sources

After three washes with PBS-0,05% tween buffer, wells were incubated for 1 hour with 200 µl of BSA diluted in PBS buffer (5%, w/v) in order to saturate nonspecific sites.

After 6 washes (ELISA buffer), wells were incubated with a 1 3,000 dilution of anti-M13-HRP antibody (GE Healthcare) in ELISA buffer + 3% BSA for 1 h.

Control wells were incubated with 0.1M NaH2PO4 buffer without NH4SCN.

After three washes in PBS + 0.05% Tween-20, wells were incubated with blocking buffer (PBS + 0.05%Tween-20Tween-20+1% serum) for 2 h at RT in the dark.

Control wells were incubated in the same buffer but without phage.

After washing with PBS once, wells were incubated with 200 μl SuperBlock blocking buffer (Thermo Scientific) at RT for 1 h.

Antigen coated wells were incubated with 100 μl of rabbit anti sera diluted (1 1000) in blocking buffer.

The test wells were incubated at 37°C for 60 minutes and washed three times with 200 μL of washing buffer.

Plates containing 250 μl of lysis buffer per well were incubated for 30 min on ice.

GM1 immobilized on the wells was incubated with Hanks buffered saline solution containing 5 μM of an FITC-Aβ(1 40) solution in the dark for 1 h at room temperature.

Following washing 3 times with 1× DNA binding buffer, each well was incubated with 100 μl rabbit polyclonal HSF1 antibody (B7109, Assay Biotechnology) diluted 1 1,000 in SuperBlock blocking buffer at RT for 2 h.