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The stainless steel electrode was then removed and the tube containing the cells and buffer was vortexed for 30 s to break up any remaining cell clusters.
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After addition of 1 ml of phosphate buffer, the solution was vortexed for 1 min and centrifuged at 4000 rpm for 10 min.
After this, 1 mmol of maghemite nanoparticles (γ-Fe2O3) were mixed with the BSA containing buffer and the mixture was vortexed for 1 h at room temperature.
Subsequently, 0.8 mL of HBS-EP buffer was added, the sample was vortexed for 10 s and vigorously shaken for 10 min, followed by a centrifugation at 2,400× g for 10 min.
After briefly centrifugation of the tube to remove drops from the inside of the lid, 200 μl of Buffer AL was added, and the mixture was vortexed for 15 seconds.
Nuclear extract buffer was added to the pellet and the sample was vortexed for 20 seconds.
The mixture was vortexed for 10 minutes before the addition of 200 μl of TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).
The mixture was vortexed for 60 s.
Reaction mixture was vortexed for 15 20 s.
The mixture was vortexed for 10 seconds.
Membrane proteins were extracted by adding 200 μl extraction buffer and samples were vortexed for 30 s.
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