Sentence examples for buffer was used with from inspiring English sources

Experiments were done as described previously (Lisov et al. 2014) except that the 75 mM universal buffer was used with pH optimum of the xylanases (i.e. pH 7.0 for CFXyl1 and CFXyl3, and pH 7.5 for CFXyl2 and CFXyl4).

A Krebs - Ringer medium buffer was used with the following composition in mM: 130 NaCl, 4.7 KCl, 1.2 KH2PO4, 1.2 MgSO4 and 2.56 Cand2 and supplemented with 1 mg/ml BSA and 3 mM D-glucose.

Modified Krebs-Ringer bicarbonate buffer was used, with the following composition: 10 mM D-glucose, 2.5 mM CaCl2, 0.49 mM MgCl2 × 6H2O, 4.56 mM KCl, 120 mM NaCl, 0.7 mM Na2HPO4, 1.5 mM NaH2PO4, and 24 mM NaHCO3, supplemented with Ficoll T-70 4% and BSA 0.1% and gassed with 95% O2, 5% CO2.

Krebs-Henseleit buffer was used throughout, with the following composition: NaCl 120.0 mM, KCl 4.7 mM, MgSO4 1.2 mM, K2PO4 1.2 mM, Glucose 11.0 mM, CaCl2 2.5 mM, NaHCO3 25 mM.

Alternatively, for glycerol studies, NP40 containing lysis buffer was used without or with increasing concentrations of glycerol as indicated.

After tagmentation, a Purelink PCR Purification Kit with HC Binding Buffer was used for purification and eluted with 30μL of EB or H2O.

An aqueous solution of 0.1 M buffer was used for each measurement with 0.2 M sodium sulfate as a supporting electrolyte.

For plasmid and IBB / plasmid binding assay analysis 0.8%% agarose gel in TBE buffer was used, and then stained with ethidium bromide.

Founder fish treated with buffer was used as the control (ENU conc., 0 mg/kg).

For each well in the 6 well plates used for cytokinesis release, 0.5 ml Mb buffer was used (∼4 × 10 cells with ∼80% of the population at cytokinesis).

For PCR amplification, Phusion® High-Fidelity PCR Master Mix with HF buffer was used (Finnzymes; Fisher Scientific, Landsmeer, The Netherlands).