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A more representative and physiological medium, pH 6.8 mHanks bicarbonate buffer, was used in this study to evaluate the in vitro dissolution of enteric coated multiparticulate-based PPI products.
The serially diluted E. tarda of several concentrations (1 × 106, 5 × 105, 1 × 105, and 5 × 104 CFU/ml) in sample buffer was used in sensitivity detection.
This buffer was used in all incubations and washes.
In this step, blocking buffer was used in place of serum.
A volume of ∼30 ml washing buffer was used in each round of dialysis, which just submerged the horizontally placed dialysis cassette in a 150 ml beaker.
Following assembly, 350 uL of DEPC-RSB200 buffer was used in the initial immunoprecipation (IP) steps via primary bead fractions Protein G Plus/Protein A Agarose suspension with PBS (Oncogene) pre-blocked with 25 µg of whole HeLa cell extracts.
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An adaptive playout buffer is used in a VoIP terminal to overcome jitter.
The ITLC test on impurity D is not required because no HEPES buffer is used in the preparation process.
Slow-exchanging amide protons identified in the 2D 15N-1HSQCQC spectra recorded after the H2O buffer was exchanged for a D2O buffer were used in the structure calculated with the NOE distance restraints to generate hydrogen bonds for the final structure calculation as previously described in the literature (Chakravarty et al., 2009).
Four isotonic buffers were used in preparing DPPC dispersions at physiological conditions for possible biological applications: (1) a standard PBS solution; (2) the above PBS with 1 mM CaCl2; (3) PBS with one tenth the previous standard phosphate salt concentrations and 2.5 mM CaCl2; and (4) 150 mM NaCl with 2.5 mM CaCl2 and 10 mM HEPES (4- 2-hydroxyethyl -1-piperazineethanesulfonic acid).
Different McIlvaine buffers were used in the pH range 3 8.
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